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The construction steps for cloning the TuMV full-length cDNA into the pBD003 binary vector are shown.
SCNT follows specific steps for cloning: an egg cell is prepared by having its nucleus removed; an adult (or somatic) cell has its nucleus removed; this nucleus is inserted into the prepared egg, which is then stimulated with an electrical shock inducing egg cell division.
Steps for cloning oligonucleotides into pSUPER.retro vector were made accordingly to pSUPER RNAi system protocol (www.oligoengine.com).com
The steps for cloning the NoV GII-4 (GenBank sequence database accession number AF080551) full-length capsid gene are described elsewhere [ 11].
In addition, the manufacturing process for biologics is more complex than for small-molecule drugs, requiring multiple steps for cloning; selecting, maintaining, and expanding the cell line; and isolating, purifying, and characterizing the product (Fig. 1).
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Escherichia coli strain MRF' XL-1 blue was used as plasmid host for cloning steps.
They attempted to adopt one step of the process used for cloning animals -- the transfer of a cell nucleus -- as part of a strategy to develop human embryonic stem cells.
In the second step, specific restriction sites were incorporated for cloning into the pHAL30 phagemid vector.
She is a perfect candidate for cloning.
Messing, J. New M13 vectors for cloning.
Finally, the NGS approach does not use a step for molecular cloning into bacteria and thus seems freer from the cloning bias than the traditional approach.
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