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Improvements in the purification procedure, especially the use of fractional ammonium sulfate precipitation steps, enabled us to obtain highly homogenous and pure IL-4 protein, giving a single protein band on silver-stained SDS polyacrylamide gels.
All of these steps enabled us to diagnose the relationship between low-energy electron fluxes and their associated magnetic perturbations with regard to their effect on LEO satellites.
Again, these selection steps enabled us to define a sample of 576 individuals from the AIBL cohort.
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Integrating a tetracycline resistance cassette in the first step enabled us to select for successful recombinants after the second recombination using growth suppression of Tetr clones on Bochner-Maloy plates [9], [10].
This preliminary step enabled us to select appropriate discriminating chemical elements which have subsequently been plotted in bivariate plots after transformation by the additive log-ratio method (alr).
This step enabled us to estimate the subject's self-representation of BMI, which can be biased by self-perception disorder [35] or by difficulties in identifying oneself with a virtual representation.
A cosmid-based gene replacement method that does not include a high temperature step enabled us to disrupt both the ssrA and smpB genes separately and at the same time suggesting that the tmRNA tagging system may be required for cell survival under high temperature.
This step enabled us to eliminate entire assemblies with large contigs not supported by any other assembly.
This analysis step enabled us to look at differential brain activation that was significantly stronger in 1 task in comparison tothe 2 other tasks.
This step enabled us to check database annotations and eliminate sequences that were impossible to align to the four human ANT genes.
This step enabled us to identify potentially problematic items and re-translate them until we were confident that the items would be interpreted in the same manner in all languages (i.e., Flemish, French, and German).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com