Exact(3)
The final washing steps consisted in three washes of 5 min in TBS-Tween buffer.
The next steps consisted in an in vitro transcription mediated linear amplification to increase the number of all mRNA molecules.
The last cleaning steps consisted in masking repeated sequences and removing chloroplast derived sequences: 1,068 Mb of high quality sequences were eventually used for de novo assembly (Table 1).
Similar(57)
The fourth and fifth steps consist in eliminating redundant or irrelevant variables, using adapted techniques of discriminant analysis.
The selection steps consist in accepting or rejecting the mutated network, in accordance with the criterion of maximization of the cost function B, in Eq. (7).
It would seem logical to add a third step after the search and destroy steps, consisting in gut re-colonisation with normal flora or E. coli.
The refolded enzyme was purified in two steps consisting in Q-Sepharose HP chromatography and gel filtration on Superose 6 Prep Grade.
The second step consisted in achieving steady-state conditions.
The following step consisted in building a 3D gridded model with seven different modeling units.
The first step consisted in improving an existing random dynamic model by determining optimal dimensions.
The second step consisted in establishing future risk scenarios which represent the possible flow and fate of CO2.
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