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The following steps consisted of introducing school-level and class-level variables into the model (model 2).
All reactions involved occurred efficiently and purification steps consisted of washing the reaction mixture with aqueous solutions and precipitation of the product in diethyl ether.
PCR amplification steps consisted of preliminary denaturation step at 95 °C for 3 min, followed by 34 cycles at 95 °C for 15 s, at 57 °C for 15 s, and at 72 °C for 1 min.
The final washing steps consisted in three washes of 5 min in TBS-Tween buffer.
The second and the third steps consisted of cloning the EYFPBax and ECFPBax cDNA.
The next steps consisted in an in vitro transcription mediated linear amplification to increase the number of all mRNA molecules.
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The three steps consist of aggregation, optimization, and control.
The sample QC steps consist of five scripts [ Fig. 1 (in light blue, see online version)].
ChIP-seq will generally have two intermediate steps consisting of read mapping and peak calling.
The explicit time for discrete steps consists of adding a real parameter to each node state.
The next step consisted in the automatic import of the street layout from OpenStreetMap.
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