Sentence examples for steps by adding from inspiring English sources

But the Jets, like the Raiders, were in desperate need of upgrading the roster, and they have taken positive steps by adding running back Le'Veon Bell, wide receiver Jamison Crowder and linebacker C.J. Mosley.

For both the participation and performance models, we built each model in several steps by adding variables.

Two further extraction steps by adding 1 volume of 37% formaldehyde into 2 volume re- suspended cells in PBS were applied.

The initial concentration was followed by two wash steps by adding 14 mL of PBS and spinning each tube at 3220 rcf at 4°C for 1 h.

The protein content was precipitated in two steps, by adding 1 vol of chilled acetone at −20 °C and vortexing for 1 min to generate fine-sized protein aggregates, followed by adding 5 vol of chilled acetone.

Further chain elongation up to 16-cabon palmitoyl-ACP is carried out by keto acyl ACP synthase I (KASI) in six consecutive steps by adding two carbons in each step.

This scenario was then further developed in the third step by adding groundwater constraints, which formed a more realistic scenario where only 8% of the impervious area was connected to soakaways and the reduction in CSO volume was 24%.

Today, they've taken another step by adding the BlackBerry Pearl to their website as "available now".

After washing with the same buffer but without Triton X-100, the inclusion bodies were collected by centrifugation at 8000 rpm for 10 min and subsequently subjected to the solubilisation step by adding of 25 ml of 8M urea and 5% β-mercaptoethanol (βME).

For screening of protein microarrays, the GST tag was removed following the final GSH-sepharose washing step by adding thrombin (GE Life Sciences) as suggested by the manufacturer, following by size exclusion filtration using a 50 kDa cut-off Microcon filter (Millipore).

We have made another step by adding cells to this type of scaffold.