Sentence examples for steps bound from inspiring English sources

Yet their warnings did not prevent the Egyptian government from taking steps bound to further infuriate the White House.

After several washing steps, bound bacteria were detached and amplified before being subjected to a new round of panning.

After washing steps, bound radioactivity was detected on a Wallac Wizard 1470 automatic γ-counter (Perkin Elmer, Waltham, USA).

After three washing steps, bound radioactivity was quantified in a gamma counter (LKB Wallac 1261; Perkin-Elmer, Waltham, Massachusetts, USA).

After a final washing step, bound proteins were analyzed as in Figure 2C.

Cell lysates were then treated with NeutrAvidin beads, and after a centrifugation step bound proteins were subjected to Western blot analysis using an anti-His antibody.

After an additional wash step bound rat IgG was detected using a horse-radish peroxidase-labelled goat anti-rat IgG antiserum (Bethyl Laboratories, Montgomery, TX) using 1-Step Ultra TM-ELISA substrate (Thermo Scientific, Rockford, IL) as chromogen.

After a final washing step, bound antibody complexes were detected by incubation with HRP-substrate ABTS (Roche, Mannheim, Germany) for 20 min at RT in the dark, followed by analysis of the absorption at 405/650 nm in an ELISA reader.

After the washing step, bound peptides were eluted either in TFA, that resulted in esterified-peptide elution, or in ammonium hydroxide, that led to the regeneration of the carboxyl group.

Following a wash step, bound ochratoxins were dissociated from the Mabs using a small volume of acidified acetonitrile/water (2/8  v/ v) prior to separation plus identification with nano-LC-Q-ToF-MS.

After a washing step, bound antibodies were labeled using a secondary antibody coupled to alkaline phosphatase (1 2000 polyclonal goat anti-mouse and anti-rabbit immunoglobulin/AP, DakoCytomation, Hamburg, Germany) over 45 minutes.