Sentence examples for steps are preformed from inspiring English sources

When these two steps are preformed there is a risk that the air bubble could exert pressure on the IOL, endangering its stability, with possible malpositioning or even dislocation into the vitreous chamber.

Tubes were beadbeat using BioSpec Products Inc. Minibeadbeater-16 for 1 min and incubated at 95°C for 10 min. The remaining steps were preformed as per manufacturer protocol.

All purification steps were preformed at 4°C.

Protein coding regions and the LTR were extracted from the reference contigs, based on the annotations from LTRHarvest [ 36] and LTRDigest [ 41] and the BWA alignment step was preformed with more relaxed settings (bwa flags: -l 1024 -i 0 -o 3).

Sectional gutters are preformed and sold in pieces.

These cytolytic effector molecules are preformed within cytoplasmic granules.

For real-time quantification of target gene expression, one step RT PCR was preformed using the QuantiTect SYBR Green RT PCR Kit (Qiagen) on an Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, Darmstadt, Germany).

This two step amplification procedure was preformed in order to avoid non-specific amplification of the CYP2D6 pseudogene located near the CYP2D6 gene.

All steps of our method were preformed manually, with the only exception of a robotic step used to normalize our PCR II samples before pooling.

One-step real-time RT-PCR was preformed as described for QuantiTect SYBR Green RT-PCR kit (QIAGEN, Valencia, CA).

The clustering was preformed in a two-step process.