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Therefore washing steps are indispensable to reduce possible background fluorescence.
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Our results demonstrate that a purification step is indispensable in addition to resin extraction in order to obtain pure methanobactin.
However, a derivatization step was indispensable for LC-ESI-MS/MS and GC MS to achieve the analysis of sugars and alditols, and the derivatization products were very complex because reducing sugars usually have varieties of isomers that co-exist in aqueous solution, which would increase the difficulty for qualitative and quantitative analysis by chromatographic technique.
This step is indispensable for broad clinical application.
32 The difficulties experienced in practice by our participating GPs led to suggestions of how to make evidence-based guidelines more viable in patients with multimorbidity a necessary step, since guidelines are indispensable in the current era, as was confirmed by the participants.
Olli Rehn, the European commissioner for economic and monetary affairs, said that although Athens had made budgetary adjustments in the last 12 months, further "decisive steps by the Greek authorities are indispensable".
All are indispensable.
Which works are indispensable?
Both worlds are indispensable.
Ferries are indispensable for transportation.
"None of us are indispensable.
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