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Systematic differences between published data sets may also result from different pre-processing steps applied by the authors.
From this analysis we found that for all patients, despite the large amount of sequences generated by the 454-Roche or MiSeq Illumina platforms, after the sequential filtering steps applied by our pipeline, the number of sequencing reads univocally mapped on the genome was progressively reduced to 30% of the initial number of sequencing reads.
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The final step applied by the authors corresponds to the statistical k-nearest neighbor (KNN) classifier implemented for different values of k (k = 1, 3, 5, 7, 9, 11, 13).
DOI: http://dx.doi.org/10.7554/eLife.10005.007 Finally, we skipped the phylogenetic curation step applied by Smith et al. to reduce the number of false positive wheat alignments, and mapped all reads sequenced by Smith et al. to the wheat genome.
The predictor step is applied by replacing the spatial and temporal derivatives by forwards difference to obtain the predicted value of h, indicated as h in Eq. 3.
The predictor step is applied by replacing the spatial and temporal derivatives by forwards difference to obtain predicted value of h, indicated as h in Eq. 7.
Firstly, a syntactic verification step is applied by using predicate logic.
The filtering step used to select only the longest transcript per gene produced 223,365 contigs, and the additional step applied to remove redundant sequences by MIRA 3.4.0 [ 40] and to filter sequences shorter than 250 bp further reduced the Trinity assembly to a set of 105,653 transcripts.
Most famous for placing a poisonous snake in the mailbox of an attorney who opposed it, Synanon was founded by an AA member who believed that the steps needed to be applied by force and that people with addiction needed to be broken down completely before they could recover.
In this paper, the kinetics of redistribution were investigated for a 2-step hydroxyapatite-based consolidant, applied by brushing and immersion.
Voltages were applied by stepping, which positively influenced passivity breakdown and played a significant role in the formation of self-organized nanoporous films.
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