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Our studies are only the first steps and quantification of costs and benefits in order to establish group foraging as a more general pattern is necessary.
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In conclusion, the two-step pathogen detection method based on CE-SSCP described here allows for sensitive detection of pathogens by multiplex PCR (first step) and quantification by specific PCR (second step).
This work described a rapid strategy for one-step extraction and quantification of the components.
After the purification step, sizing and quantification of all the generated libraries were measured again on the Agilent 2100 Bioanalyzer (Additional file 2: Figure S3 B) shows a typical electropherogram after purification).
The program used for amplification was: denaturation (95°C for 15 min ., 2-step amplification and quantification (92°C for 15s, 60°C for 1 min. and one fluorescence measurement), melting curve program (60 90°C with a heating rate of 0.1°C per second and one fluorescence measurement per second).
Most of current RNA-Seq tools for RNA-Seq quantification contain two main steps: an alignment step and a quantification step.
The high reproducibility in expression levels avoids protein purification and quantification steps for false positive variants and avoids real positives from being discarded.
The reproducibility of the quantification results obtained from the different samples demonstrates the robustness of the extraction and quantification steps (Table 3).
Subsequent detection and quantification steps were as described above.
The amplification, detection and quantification steps were carried out using the Rotor-Gene 6000 centrifugal thermal cycler (Corbett Research Mortlake, Sydney, Australia).
We therefore combined both the published and reprocessed data and performed ANOVA with the new factor 'quantification' that represents the mapping and quantification steps.
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