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The method of steps allows one to solve virtually any DDE system by transforming it to an ODE system.
Although the nominal spatial resolution of MR images is inherently lower than that of optical methods, we demonstrate that the digital resolution achieved after the image processing steps allows one to determine the spatial positions of the inner and outer contours with 50 μm precision.
Cycling the phase(s) of the excitation pulse(s) preceding the indirect dimension in a 2D NMR experiment in three (or more) steps allows one to select either coherence order p=1 or p=−1 by changing only the receiver phase.
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We demonstrate that specificity, expression kinetics, and protein design are crucial parameters for efficient gene repair and that our two-step assay allows one to go quickly from design to testing to successful employment of the custom nucleases in human cells.
The step-by-step analysis method allows one to comprehend the structural behaviors of the bridges during the construction, service, and failure periods, respectively.
When performed under an error-prone condition with Taq DNA polymerase, PPCP allows one-step construction of mutagenesis libraries based on in situ error-prone PCR so that random mutations are introduced into the target gene without altering the expression vector plasmid.
The application of NOTA-AlF chemistry chemistry and kit formulation allows one-step 18F-labeling.
The plasmid pASK-IBA3 encodes for a C-terminal Strep-Tag that allows one-step purification of the recombinant fusion proteins using Strep-Tactin-Sepharose [43].
The dual acetylation of Lys9 and Lys14 along with Ser10 phosphorylation allows one-step higher level of mechanism for controlling gene regulation.
For high throughput subcloning of short peptide genes, we constructed the vector, pET30a/His-EDDIE-GFP, and used a seamless enzyme-free cloning method for high level expression of AMPs in E. coli, This method allows one-step assembly of DNA fragments in vivo via homologous recombination in E. coli [ 24].
Two step photocatalytic tests, i.e. a cleaning step in the presence of water vapour followed by purging of the gas phase and a second step after CO2 addition, allow one to discern between the carbon-containing gaseous products originated from carbon impurities and those produced in the presence of CO2.
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CEO of Professional Science Editing for Scientists @ prosciediting.com