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We'll break this tutorial into three steps: acquisition, conversion, importing.
'TOM software toolbox' integrates established algorithms and new concepts tailored to the special needs of low dose ET. It provides a user-friendly unified platform for all processing steps: acquisition, alignment, reconstruction, and analysis.
Determining the 3-D structure of a sample from electron tomography involves three major steps: acquisition of sequence of 2-D projection images of the sample with the electron microscope, alignment of the images to a common coordinate system, and 3-D reconstruction and segmentation of the sample from the aligned image data.
SPECT/CT scans were performed with MEGP and HEGP collimators using the same protocol (matrix, 64 × 64; pixel size, 8.8 mm; scan orbit, body contour; frames per detector, 30 (6° steps); acquisition time per frame, 60 s; total acquisition time, 30 min).
The quantitative analysis involves the following steps: acquisition of the image by AxioVision software; processing and quantitative measurement of the stained fibrous areas using a specific protocol of AxioVision software for the quantitative analysis with an automated thresholding used for all the slides of each staining; percentage ratio between the fibrotic area and the entire sample.
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The two-step acquisition consists of two master acquisition processes.
The two-step acquisition time is given by (12).
Shown as the curves, the two-step master acquisition requires much fewer feedback times than single-step acquisition.
With a big, the feedback times of two-step search is even less than that of the single-step acquisition.
The proposed two-step acquisition time is much less than that of the single-step scheme as expected.
In the proposed two-step acquisition, the optimum pulse width transmitted by master node in the first step (12). is chosen by minimizing acquisition time.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com