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Exact(6)
Current-voltage curves were obtained by stepping the voltage from the holding potential to voltages of −80 to +50 mV in 10 mV increments.
Cells at a holding potential of −90 mV were subjected to 4 s depolarizing steps to voltages in the range −70 to +50 mV before stepping the voltage to −70 mV where tail currents were recorded.
The currents were elicited by first stepping the voltage to +60 mV for 5 s then to −60 mV for 3 s tails.
The currents were elicited by first stepping the voltage to +60 mV for 5 s then to -60 mV for 3 seconds tails.
Half activation voltages were calculated using the peak current achieved by stepping the voltage for a duration of 500 ms from −60 mV in 10 mV increments every 10 s to +110 mV.
By stepping the voltage in one cell (cell 1) while keeping the potential of cell 2 at Vh, thus establishing a transient transjunctional voltage Vj ≡ V1 − V2 = Δ V1, junctional current (Ij) was measured directly as the current change in the unstepped cell (i.e. Ij = −Δ I2).
Similar(54)
To quantify KCNQ2/3 currents, we held fusiform cells at −30 mV for 5 s and then stepped the voltage to −50 mV for 1 s to unmask the slow deactivation of KCNQ2/3 channels.
We stepped the voltage V0 of the patch clamp amplifier connected to one cell (cell 1, Figure 1A) in 10 mV increments from the zero current potential (−61±2 mV, n = 15 cells) while monitoring the membrane potential (V m ) of a nearby cell (cell 2, Figure 1A) maintained under current clamp conditions with a second amplifier.
Stepping the membrane voltage from −140 mV to +60 mV, produces constitutively open channels whose currents reverse at the expected reversal potential for K+ (about −85 mV, Figure 2F).
Transformer, device that transfers electric energy from one alternating-current circuit to one or more other circuits, either increasing (stepping up) or reducing (stepping down) the voltage.
Both use mainly DC/DC converters to step down or step up the voltage to provide regulated voltage.
Related(16)
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