She should take care not to invest every step with the same intensity: the role has fine shadings as well as bright sunshine.
The results shown in this section were computed using one "in place" processing step with the same input frames as before (i.e., t−1, t0, t1) to further stabilize the results, followed by an iteration including a new frame (i.e., t0, t1, t2).
Strips were equilibrated in 6 M urea, 2% SDS, 30% glycerol, 0.375 M Tris-HCl pH 8.8, 0.002% bromophenol blue and 10 mg/ml DTT for 15 min, followed by a second equilibration step with the same buffer containing 25 mg/ml iodoacetamide instead of DTT, also for 15 min. Strips were loaded on 12% SDS-gels with an overlay of agarose solution (0,5 ml/100 ml electrophoresis buffer).
Since transcription initiation in this promoter has at least two rate-limiting steps, the fluctuations in RNA numbers will be weaker than if there was only one rate-limiting step with the same total mean duration, exponentially distributed.
This method is similar to the D > C criterion as described above, except for an additional reverse BLAST step with the same parameters to confirm that the two sequences are each other's best match within their host genomes.
It should be noted that the COMP threshold and the weight combination in the calculation of the COMP value were determined in the optimization step with the same data that was used in this discrimination step, due to a small number of entries available.
(Ms. Osipova's feet, though clad in noiseless blocks and giving her an unequalled jump, do not yet trace small steps with the same kind of delicacy).
The algorithm runs steps with the same temperature.
In this version, Breslin —although she tried — just wasn't hitting those mambo steps with the same gusto Jennifer Grey did. .
The length of a block was the number of consecutive steps with the same step length.
Thus larger time steps with the same random distribution cause greater variation in the protein concentration.
