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The first step was achieved by reducing gold chloride ion in presence of AuNP acting as nucleation seeds.
With the adoption of core Sensor Web standards as part of the INPSIRE Technical Guidance another significant step was achieved in the year 2016 [5, 6].
The cyclization step was achieved by condensing the N-chloroacetyl derivatives with the appropriate anilines in presence of phosphorous oxychloride in dry toluene.
This last step was achieved within 1 h, a much shorter duration compared to conventional routes which usually take several hours to few days.
With the GPU program, a maximum performance of 56 GFlops (3.3 ms per 106 unit cells per time step) was achieved for a FDM domain with a size of 384 × 384 × 384 (about 2.7 GB of memory).
Washing step was achieved by using wash buffer (50 mM NaH2PO4, 300 mM NaCl, 20 mM imidazole, pH 8) while purified recombinant bromelain was eluted using elution buffer (50 mM NaH2PO4, 300 mM NaCl, 500 mM imidazole, pH 8).
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The most challenging step was achieving timely registration in the user countries.
In the synthesis of cADPtR (3), the key step was achieving stereoselective construction of the N1-β-thioribosyladenosine structure.
In Algorithm 2, this step is achieved by the function (isLeader (line 3). 2.
This step is achieved using the SF-HBM in conjunction with ALC.
This step is achieved by the surface functionalization of SWNTs using a mixture of strong acids H2SO4:HNO3(volume ratio 3 1) [32].
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