Sentence examples for step tissue from inspiring English sources

For the second step, tissue constructs are engineered using uniaxial or equibiaxial static constrained culture conditions.

After the blocking step tissue was incubated with the primary antibody for 72 h at 4°C, and then washed for three times with PT for 20 minutes prior to the addition of the secondary antibody.

Tissue fragments of approximately 0.5 × 0.5 × 0.5 cm were submitted to mechanical dissociation in Petri dishes: after a medium washing step, tissue sample was first finely cut with a scalpel blade and then crushed with a striated plunger from a disposable syringe.

Between each step tissues were washed with PBS several times.

Following each labeling step, tissues were washed every 10 minutes for 30 minutes with PBS containing 0.1% saponin.

Following intensive washing step, tissues were incubated with a rat anti-human CD31 (anti-PECAM1; Millipore, Germany) for 2 h, followed by incubation with a goat anti-rat IgG-Cy5 conjugate (Milipore) for 90 min, all within the previously described solution (nonfat milk/PBS/Triton).

Beyond cartilage repair, this functional biphasic scaffold may provide a biomaterial framework for one-step tissue engineering strategy by homing endogenous cells to stimulate tissue regeneration.

Between all subsequent steps, tissue was rinsed with TBST (4 × 15 min) unless otherwise noted.

An abbreviated protocol followed from MBL International Corporation (www.mblintl.com) included the following steps: tissues were isolated and snap frozen in liquid nitrogen.

Cell seeding of 3D scaffolds is a critical step in tissue engineering since the final tissue properties are related to the initial cell distribution and density within the scaffold unit.

Herein, we provide evidence that the fusion of cellular bioink (a critical step in tissue maturation) can be accelerated by higher apparent tissue surface tension (ATST) of the cellular bioink particles.