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In this step, terminal uridylyl transferases (TUT2/PAPD4/GLD2, TUT4/ZCCHC11, and TUT7/ZCCHC6) specifically mono-uridylate the 3′ end of the group II pre-let-7s, yielding the two-nucleotide 3′ overhang preferred by Dicer (Heo et al., 2012).
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Family members signed up to climb Cleveland's 804-step Terminal Tower for the American Lung Association and put the plunge proceeds towards the larger goal of raising more than $1,000 for the cause.
Lung alveolar cell apoptosis was detected using the one-step terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) apoptosis assay kit (Beyotime, Shanghai, China) according to the manufacturer's instructions.
According to Table 1, if the reactive power remains unchanged and the active power is adjusted step-by-step, the terminal voltage mainly displays amplitude variation.
In a second step, one terminal is used to apply a sinusoidal current while the other terminals are used to monitor the local potential distribution at different positions along the electrodes of the cell.
PTGR2 catalyzes an NADPH-dependent reduction of the conjugated α,β-unsaturated double bond of 15-keto-PGE2, a key step in terminal inactivation of prostaglandins and suppression of PPARγ-mediated adipocyte differentiation.
In the last step, the terminal aromatic anion may abstract a proton from t-butanol to give the final product.
In the first step, the terminal –OH groups on the surface of silica nanoparticles were converted to –NH2 groups by the self-assembly of γ-aminopropyltriethoxysilane on the surface of the silica nanoparticles.
For every time step, the terminal velocity is evaluated by Eq. 9. To avoid numerical errors, the time step is adjusted to make the space step constant, which means near regions with high field strength, the time step is effectively reduced.
In a subsequent step, the terminal glutamate is converted to a pyroglutamate in a dehydration reaction.
In contrast, BN82865, which inhibits the second transesterification step in terminal spliceosome processing, did not have this effect.
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