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After the weighing step, steps 3 to 9 of the QuEChERS method (Table 4) were executed.
Cycling was done in a Fast System 7500 Real Time PCR Cycler (Applied Biosystems) according to the following program: step 1: 10 minutes at 95°C; step 2: 15 seconds at 95°C; step 3: 30 seconds at 55°C; step 4: 45 seconds at 60°C (data collection was performed at this step); steps 2 to 4 where repeated for 40 cycles.
Then, the second step (steps 5 to 7 of Algorithm 2) is performed on STATA and consists in regressing the bias-corrected technical inefficiency scores over a set of exogenous covariates using a bootstrapped truncated regression with 1,000 iterations in order to obtain unbiased coefficients and confidence intervals.
Key steps: Step 1: input original image.
It consists of 6 steps: Step 1: Palm to palm.
The protocol included the following seven steps: Step 1.
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Step, balance, step, balance, step, step, step, step.
No more step, step, step, leap.
Step! Step! Step!" Up six steps; down six steps; through ten doors.
The Fine Art Of Shucking, Step by Step STEP 1: Wear gloves.
Step, kick, step, step curtsy, turn to left, arms out, then kneel.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com