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Between each step, sections were washed with PBS, 0.1% Triton-X100.
After washing step, sections were reacted with 3,3′-diaminobenzidine (DAB) for 10 min and counter-stained with Mayer's hematoxylin.
Coronal step sections of the brain were made at the five following levels- olfactory bulbs, cerebral cortex, thalamus, midbrain and medulla.
After a blocking step, sections were incubated overnight in chicken polyclonal anti-BDNF antibody (1∶400, Promega), then exposed to the biotinylated donkey anti-chicken IgG (1∶200, Promega) followed by extravidin FITC (1∶300, Sigma Aldrich).
Quantitative morphometric analysis of remyelination was conducted by sampling remyelinated axon density in step sections collected every 0.25 mm along the rostral caudal axis of the nerves and integrating across distance essentially as described previously [34].
After each step, sections were washed with PBS.
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Detailed documentation of changes is included in the Process Step section of the metadata.
The proposed energy harvester is designed with a piezoelectric cantilever beam which has a step section.
Basis vectors and are shown for use during the modeling step (Section 7).
In some cases, the normalization step (Section 2, point 5) results in negative values.
During this step, sectioning of the medial collateral ligament is performed (Fig. 1).
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