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A step by step sample calculation is also shown for practical use of the proposed methodology.
In this step sample times are computed and summed, a call-graph representing observed function calls is built, and per-node and per-edge information is created.
Since barcode sequence tags can be added during this step, sample multiplexing is possible [ 15].
Since barcoded adapters can be added to the mixture in this step, sample multiplexing is allowed.
This was followed by a protein denaturation step (sample heated twice at 100 °C for 5 min).
For this last step, sample size was expanded to include 88 additional individuals (41 females) without Geolocator data but that were nonetheless monitored for breeding progress.
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In a further step, samples were produced by compression moulding using the compounded composites.
After each pressure reduction step, samples from all phases (oil, condensate, free and associated gas) were collected and analysed.
At this step samples were dehydrated.
After this step, samples were evaluated for in vivo biological activity.
At the end of each step samples were withdrawn from blood and dialysiscircuit, and CO2 removal (VCO2) was measured.
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