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During training step the minimum value of RTT is not observed, while it is during verification step, resulting in a short predicted distance.
In this reaction, Cp2TiMe2 is treated with [Ph3C][BF20] (TBF20) in the first step resulting in a mixture of species which are assigned as [Cp2TiMe(CD2Cl2)][Cp2TiMe][BF20]e][BF20] and [(Cp2TiMe)2 μ-Me)][BF20].
After 1 h of incubation, the labelled diabody was separated from unbound activity by a gel filtration step resulting in a final radiochemical purity of >99% (Additional file 2) and a corresponding specific activity of 4.2 MBq/μg (231 MBq/nmol diabody).
Depending on its protonation state, the enzyme has different substrate affinities and speeds of the rate limiting substrate conversion step, resulting in a pH dependent transition between different Michelis-Menten like substrate saturation behaviours.
Biotin-16-dUTP was incorporated during this step, resulting in a biotinylated cRNA (complementary RNA) probe suitable for hybridisation.
Individual spectra were acquired after each scanning step resulting in a complete map of the spectrally-resolved intensity distribution of the samples in reflection or transmission.
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However, for the octadecanoate, decomposition occurred via a single step resulting in an overall mass drop of 71%%.
Therefore, DNA exchange is normally limited to a single 180° rotation step, resulting in an unknotted inversion product.
Second, CQ can disrupt lysosomal function and prevent completion of autophagy at the final step, resulting in an accumulation of LC3-II [27].
The optimization step resulted in a (1025%) reduction in the number of faces for the models.
This screening step resulted in a pool of 154 residues (of 327 positions in the original alignment) to choose from.
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