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One hundred and fifty microlitres of PBS at pH 7.4 (Gibco, Life Technologies) was added to the bead, briefly mixed by vortexing at high speed, and removed (step repeated once).
Saudi Arabia has ordered Iranian diplomats to leave the country after severing diplomatic ties, a step repeated by several allies, while Iran defended its response to the embassy attack and said 50 people had been arrested.
A series of three steps of FFD-SF operations (with each step repeated, and there were 6 steps in total) yielded separation of the sea sediment into four fractions having diameter ranges of larger than 10 µm, between 5 and 10 µm, between 2 and 5 µm, and smaller than 2 µm.
As a next step, repeated imaging and clinical observations over a long time period would be necessary in order to appreciate the predictive value and the clinical relevance of the (discrepant) findings in both imaging modalities described, including their impact on patient management.
Seeds of P. japonicum were disinfected by washing several times with sterile distilled water, followed by dipping in 70 % ethanol (v/v) for 10 s, then immersing in a solution of 0.5%% (v/v) sodium hypochlorite containing 1 drop of Tween-20 for 5 min and the step repeated three times.
Thus, the second step repeated the simulation in a model which lacked H30, to confirm that the observed microdomain is not a result of disruption of normal cellular signaling or unbalanced FRET sensor expression.
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It was found that multi-step repeated processing in the parameter-controlled RF environment could increase nanopillar height to ∼10 μm, a 400% improvement, while the RF plasma processing with identical total duration but in a single step did not lead to desired nanopillar elongation.
Reach, pull, step and repeat.
"Step and Repeat," at the Museum of Contemporary Art.
In neurons from dopamine-depleted rats, the current-firing frequency plot is shifted to the left with a firing frequency significantly increased for each current step (repeated-measures ANOVA, p<0.05; Fig. 2B) as shown for the representative 120 pA-evoked spike response (4±0.6 spikes for untreated control rats, n = 8 and 6.12±0.7 spikes for dopamine-depleted rats, n = 8, p<0.05; Fig. 2A).
In neurons from 6-OHDA lesioned rats, the current-firing frequency plot is shifted to the left with a firing frequency significantly increased for each current step (repeated-measures ANOVA, p<0.05; Fig. 3B) as for a representative 120 pA-evoked spike response (3.28±1.35 spikes for unlesioned control rats, n = 7 and 10.9±1.93 spikes for 6-OHDA lesioned rats, n = 10, p<0.01; Fig. 3A).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com