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While this pooling of fractions from sucrose gradients precludes drawing conclusions on the regulation of translation elongation or termination, it allows analyzing translational regulation at the step of translation initiation.
A key step of translation is recruitment of initiation machinery to the 5′ mRNA cap.
Aminoacyl-tRNA synthetases (aaRSs) are enzymes that function at the first step of translation, catalyzing the conjugation of amino acids to their cognate tRNAs for protein synthesis.
Eukaryotic elongation factor 2 (eEF2) binds GTP and mediates the translocation step of translation elongation.
We show that non-AUG codons, such as CUG, engage ribosomes during the initiation step of translation.
In these models, eye position could not be held in its new position, after a step of translation.
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These observations and our results suggest that RACK1 functions as an evolutionarily conserved molecular adaptor on ribosomes to recruit a variety of regulators of mRNA translation such as miRISC and facilitates their interactions with the translational machinery at diverse steps of translation.
Since the N-terminus of bL27 inserts into the peptidyl transfer center, the addition interactions between bL27 and H31a might affect certain kinetic steps of translation elongation on the mycobacterial ribosome.
Methionine analogs provide an opportunity to investigate the substrate specificity of the initiation steps of translation.
Recently it was shown that the mammalian PABP is involved in key steps of translation initiation and might well be regarded as a canonical translation initiation factor [25].
Streptomycin is an antibiotic agent that binds to the 30S ribosomal subunit and interferes the initial selection and proof-reading steps of translation [43].
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