Sentence examples for step of the template from inspiring English sources

By adding a PNKP-triggered 5′-phosphroylation step of the template DNA, the above sensing strategy could be easily extended to the design of PNKP sensor.

The final step of the template fabrication is the electric passivation of the pore walls by an 8-nm-thick layer of Al2O3 deposited by atomic layer deposition (ALD) to avoid unfavorable current flow through the pore walls during galvanic deposition.

The equation for DE is given below: where Δ θ i n = θ i n − θ i 0 (MN ) is the imposed deviation of the ith dinucleotide step parameter θ i n at the nth step of the nucleosome template from the rest state value θ i 0 (MN ) of the step MN.

Negative controls included omission of reverse transcriptase at the cDNA synthesis step and omission of the template at the PCR step.

cDNA samples were analyzed both for the genes of interest and reference genes (β-actin) The amplification program consisted of a preincubation step for denaturation of the template cDNA (5 min, 95°C), followed by 40 cycles consisting of a denaturation step (30 s, 95°C), an annealing step (30 s, 60°C) and an extension step (30 s, 72°C).

The paper reported a new template synthesis of nickel/cobalt layered double hydroxides (Ni/Co-LDH) without any adscititious alkali source, oxidant and step for removal of the template.

The DNA was stored at −20 °C for the next step of the study as template for CPA.

Therefore, the inclusion of a preheating step in the template preparation is crucial for the accurate mtDNA measurement.

For the linear reaction, a total PCR reaction volume of 20 μL was prepared containing 10 μL of prime STAR Max DNA polymerase (TAKARA Japan), 5 μL of primer F536 (100 pmol), 1 μL of the diluted PCR product from the first step as the template and 4 μL of ddH2O.

For this reaction, a total PCR reaction volume of 20 μL was prepared containing 10 μL of prime STAR Max DNA polymerase (TAKARA Japan), 1 μL of primer F772 (20 pmol), 1 μL of primer LAC1 (20 pmol), 1 μL of the diluted PCR product from the second step as the template and 7 μL of ddH2O.

Initially, the first step in the template independent addition of G-1 catalyzed by Thg1 was compared to the adenylation reactions catalyzed by the tRNA synthetases that result in release of AMP [ 7].