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This integration is carried out with a fixed time step of size 2.5 days.
This method simply consists in oversampling the projection of our function on the basis constituted by the N initial harmonics, from a spatial sampling step of size L N to a spatial sampling step of size L M : f [ n ] = ∑ k = − M / 2 M / 2 f ˆ [ k ] e i 2 π M k n, g [ n ] = ∑ k = − M / 2 M / 2 g ˆ [ k ] e i 2 π M k n. (25).
If the conditions are fulfilled, the probability that the reaction takes place during the next time step of size Δt is determined.
Briefly, synaptic vesicles were released from synaptosomes by osmotic lysis, and were further purified by rate-zonal sucrose gradient centrifugation and a final step of size exclusion chromatography on controlled pore glass beads.
A second step of size exclusion chromatography was carried out on the previous protein enriched fractions.
However, due to our additional step of size selection and technical bias for smaller fragments by the Illumina procedure, the expected coverage per observable position was substantially greater.
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Ta0 was rapidly purified to homogeneity by a single step of size-exclusion chromatography.
Using the numeric CSP approach, static and dynamic requirements can be coupled in the same step of sizing.
The first step of ammonium sulfate fractionation of the cell lysate, where ∼90% of the target protein precipitated in the 0 25% fraction (Figure 1A, lane 2) was followed by a second step of size-exclusion chromatography using a Sephacryl – S300 gel filtration column.
EGFR-TKD protein was then subjected to a final step of size-exclusion chromatography using a Superdex 200 column (GE Healthcare) equilibrated in 20 mM Tris/HCl (pH 8.0), containing 150 mM NaCl and 2 mM DTT.
TrkA498 796 was then applied to a HiTrap butyl-Sepharose HP column (GE Healthcare) in 25 mM Mes (pH 6), containing 150 mM NaCl and 2 mM DTT, eluting with a gradient from 0.8 M to 0 M (NH4 2SO4, and subjected to a final step of size-exclusion chromatography using a Superdex 200 column (GE Healthcare) equilibrated in 25 mM Mes (pH 6), containing 250 mM NaCl and 2 mM DTT.
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