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The first step of method development consisted in searching for a suitable set of RP-conditions via the screening of a large number of RP-systems (made up of different stationary phases and/or mobile phases and/or temperature).
Examination of the compounds by HPLC (C18 reversed-phase column) coupled with a single band UV Vis spectrophotometer and electrospray mass spectrometer was the second step of method optimization.
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From the training step of methods 3 and 4, Fig. 3 visualises the found average Pearson correlations between fully delineated ICV and estimates using different position indices.
While a maintaining an exam environment as mentioned in "Final Call" step of Method-1.
On the basis of these results, a single extraction with 5 mL of the DCM/methanol mixture was used in the subsequent steps of method development.
Get people the same way you do in step 2 of method 1.
When you are finally done, follow step 6 of method 1 and go adopt more.
In step 3 of Method 1, don't fill in all the small shapes.
Do the same as in step 4 of method 1. Go to Media Generators then go to "(Legacy) Text".
Have a number in mind before you get to step 10 of method 2. Give them some time.
In step three of method three, you can have them add or subtract any number you want.
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