Sentence examples for step of heating from inspiring English sources

All antibodies required a pretreatment antigen-enhancing step of heating the slides in 95°C 0.1 mol/L citrate buffer at pH 6.0 for 10 minutes before incubation with the primary antibody.

(3) The QIAamp Stool Mini Kit procedure included the manufacturer's optional pre-treatment step of heating samples in a water bath at 95°C for 10 min, with a prior modification of pre-filling tubes with glass beads and ASL buffer, and disrupting the cells using a Precellys®24 ribolyser at 5500 cycles per min for 30 sec.

The optional step of heating the lysis solution to 65 °C was used to maximize RNA yield.

It is thus clear that the extraction phase should not be conducted using a routine process and must comprise a supplementary step of heating to 90°C for 1 h to improve bisulfite conversion.

From thermal analysis, the addition of kenaf fibre reduced the degree of crystallinity of the r-PP/PE but an improvement can be observed after the second step of heating and cooling.

An aliquot of 3  μg total RNA in 100  μl diethyl pyrocarbonate (DEPC) water was processed through serial steps of heating at 65 °C for 5 min, cooling on ice for 5 min, incubating for 1 h in a solution at 4 °C, and purification by Microcon 30 before RT-PCR.

The antigen retrieval step consisted of heating the slides in EDTA buffer (pH 7.8) at 95 °C for 45 min.

The standard amplification program included 40 cycles of two steps each of heating to 95°C and 60°C.

Samples were prepared through the solid state reaction (SSR) technique by addition of CoFe2O4 nanoparticles during the last step of heat treatment.

Since the gas side heat transfer coefficient was the limiting step of heat transfer through the vessel wall, the overall heat transfer coefficient could be estimated using the power consumption per unit volume and the liquid filling volume.

The mechanism of thermal transfer from compost to inner surface of structural layer, as a first step of heat loss, was important for improving insulation performance, which was divided into conduction and convection and discussed specifically in this study.