Sentence examples for step nested from inspiring English sources

Three microliters of RT-PCR reaction were added to the second step (nested) PCR and amplified for other 30 cycles, using the same cycling conditions of the first step.

The cox 1 sequences were amplified using two step nested PCRs.

Fluorogenic-probe based qPCR analysis now offers sufficient sensitivity to quantify ITS2 variants at the sub-clade level, and has been used to quantify polymorphic ribotypes within the individual dinoflagellate genome through the use of a PCR pre-amplification step (nested qPCR; e.g. [ 51]).

In this study, WSSV DNA was detected in pond soil samples using a 2-step nested PCR.

Using plasmid DNA (pET 100) containing the 211 bp target WSSV sequence, analytical sensitivity showed that the 2-step nested PCR protocol was able to detect down to 0.015 fg of the plasmid DNA, or approximately 2 copies of the target DNA sequence.

A two-step nested RT-PCR was carried out on all initial serum samples according to Lanciotti et al. [19].

DNA from all subjects was subjected to a two-step nested PCR protocol to isolate NAT2 from the paralogous NAT genes [8].

Thermocycle was: denaturation at 94°C for 5 min, followed by 40 cycles of denaturation at 94°C for 30 sec, annealing at 51°C for 30 sec and extension at 72°C for 30 sec, with a final extension of at 72°C for 5 min. PCR amplification of complete gag of HIV-1 was performed with two sets of primers in a two-step nested PCR strategy.

After bisulfite treatment, the promoter region of ADAMTS19 was amplified by a two-step nested PCR.

Chikungunya virus was identified from patient serum using a two-step nested PCR to amplify the capsid region of CHIKV originally described by Porter and others.

This number of somatic insertions is clearly a minimum, and one wonders how many more insertions would have been detected with two-step, nested PCR.