To this end, the teacher adjusts S6's response 'It's part of Step 1' (labelled as tracking) before affirming him, so the class gets more precise information.
It has three main steps, the first one being sometimes preceded by a preliminary step (labeled "0" below) used to reduce the number of sounds to be tested in the first step.
When optimized deconvolution calculation is completed, deconvolved data will be added to the currently selected sample as a treatment step with the string "dcn" at the end of the treatment step labels (see Fig. 5a).
Single α7-nAChRs were tagged in living cells with biotinylated α-BT and, in a second step, labeled with streptavidin-QDs.
This second step labels both intracellular and extracellular sporozoites.
The two step labeling strategy is schematically summarized in Figure 4A.
In the second step, labeling of the purified amine-modified DNA was achieved by incubation with amine-reactive dye (see the manufacturer's instructions for detailed information).
In contrast, hybridizations of target LMW RNA prepared without an amplification step (labelled-cDNA) had lower signal intensities, but little detectable non-specific hybridization to control spots; in this case the background correction step was omitted.
Following another wash step, labelling was achieved by incubating for 1 h with 0.12 μg ml−1 tetramethyl rhodamine isothiocyanate (TRITC -conjugated phaloidin in PBS aTRITC -conjugatede in the dark.
In general, F-fluoroalkyl radiolabels can be introduced using either a direct [F]fluorination of a suitable precursor-compound (already containing a leaving group at the alkyl side chain) or via two step labelling reaction using an F-fluoroalkylating synthon.
The derivatization reaction itself is a base-catalyzed Michael-type addition involving a two step labeling process in which Michael donor molecules of the labeling reagent are formed and consecutively added to the reducing end of the glycan with a stoichiometry of two label molecules per glycan (II in Fig. 2b; [ 50]).
