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A step is detected when A norm reaches a local maximum higher than a given threshold A 0. By setting the sampling frequency as f s and number of samples between two adjacent maximum as Δ N, the time duration of the kth step is ({t_{k}} = frac {Delta N}{f_{s}}) and the corresponding estimated speed is {v_{k}} = frac{P_{k}}{t_{k}} = frac{{P_{k}}{f_{s}}}{Delta N} (9).
With WT Pol I KF) and a nonextendable DNA, this step is detected in the stopped-flow instrument as a rapid fluorescence increase, attributed to unstacking of the T +1)2-AP from its immediate neighbors, whose rate and amplitude are dependent on the concentration of the complementary dNTP.
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Duffy says the next step is detecting bigger and better black hole collisions.
Dr Duffy says the next step is detecting bigger and better black hole collisions.
During the measurement only one step was detected (rapid decrease of the frequency follow by a stabilization) that could be related to a formation of a monolayer.
The RLF/INSL3 eluted at each purification step was detected by dot-blot and/or Western blot analysis.
Steps were detected from the displacement records using a custom MATLAB (The MathWorks) program (Qiu et al., 2012 ).
These steps were detected manually by using a time interval slider along the 2D trajectory and checking for changes in positional fluctuations.
For each technical replicate, artifacts in area increase between time steps are detected and visualized using a kernel density estimator (Supplementary Fig. S5).
After a second incubation and an additional washing step, the bound conjugate is detected by reaction with 3,3′,5,5′-tetramethylbenzidine 3,3′,5,5′-tetramethylbenzidine
In the human detection step, humans are detected using two methods independently.
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