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Six DMSO-based vitrificant solutions (V1 V6) were tested using a 6-step incorporation protocol.
Protein transfer allows for controllable expression of functional protein on the periphery of cells in an easy, time-efficient manner, and can be performed in either a direct one-step incorporation method or an indirect two-step incorporation method.
A micromixing model involving two steps (incorporation of bulk fluid and exchange between smaller eddies) has been used for the interpretation of the experimental data.
We describe a robust and cost-effective methodology for oligonucleotide barcoding via one-step incorporation into PCR products suitable for Illumina sequencing by standard workflows.
In the early months of 2016, we got bold enough to take the first step toward incorporation with the state of Montana.
"Do you support the act of self-rule of the People's Republic of Donetsk?" Some people interpret it as a vote for more local powers, some for broad autonomy within Ukraine, some for independence, others still as a step towards incorporation into Russia.
Future research has to scrutinize the observed processes including each step of incorporation and digestion, and how nutrients gains relate to energy expenditures and possibly loss of cell content.
Acetyl-coa carboxylase (ACACA) plays a pivotal role in the regulation of fatty acid (FA) metabolism, which mediates the first committed step for incorporation of acetate carbon into FA [32].
The final step of incorporation, step 4, may take days or weeks.
The final step was incorporation of Rpn12 marking the seal of proper lid [ 39, 60].
The first step involved incorporation of all primer pairs into two PCR mixes and amplification for 20 cycles in a conventional thermocycler.
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