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After digestion, peptides were extracted from gel pieces using step by step extraction with acetonitrile gradient (15%60%% acetonitrile with 1% trifluoroacetic acid).
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Go step by step.
A step-by-step approach using different extraction techniques while performing an ongoing risk benefit analysis, as described previously, was routinely applied.
Compared with conventional artificial step-by-step fitting approaches, the proposed extraction methodology automatically tracks the shape variation of current voltage (I V) curves and examines the first derivative of I V curves; therefore, highly accurate results can be obtained directly.
To our knowledge, this is the first practical approach for developing a quick step-by-step REA procedure that includes extraction of good quality and adequate quantity of DNA from a small volume of infected A549 cells, fast digestion of DNA, and rapid electrophoresis of the cleavage products.
The step-by-step protocol includes isolation of these cells, extraction of nuclei, fixation, restriction digestion, ligation, and whole genome amplification.
To demonstrate a step-by-step approach to the use of the operating microscope for onco-testicular sperm extraction.
Crossover from one extraction technique to another was performed when necessary as part of the step-by-step approach used.
Like your recipes step-by-step?
"This is a step-by-step process.
"It was a step-by-step thing.
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