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The anti-HBc IgM assay is a class-capture two step assay configured for detection of IgM anti-HBc antibodies during acute HBV infection.
The development of a simple two step assay for microbial DON degradation in 96 well microtiter format and its testing with the DON detoxifying bacterium BBSH 797 is reported.
In addition, Taqman probe based quantitative PCR was performed on a few selected genes using the 7500 Fast Real-Time PCR System (andlied Biosystems), and quantification of mRNA was performed in a single step assay (both RT and PCR steps carried out in the same tube) according to the Verso™ 1-step QRT-PCR low ROX kit (Thermo Scientific).
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Moreover, the two- or more step assays are cost-ineffective [ 15].
We describe a one-step assay for analyzing HLA-DQB1 alleles which are associated with susceptibility to type 1 diabetes.
They were evaluated initially on RNA templates from virus-infected cells using a two-step RT-PCR protocol that was further advanced to a one-step assay.
The performance of the one-step assay is illustrated for interferon alpha2 (IFNα2) used widely to treated chronic hepatitis C (HCV) infection and neoplastic disease.
'Two-step assay' with UDP-glucose dehydrogenase as coupling enzyme in the presence of NAD ensured epimerase activity of the monomer.
Together, these findings indicate that a one-step assay format can greatly reduce the effect of coating concentration variation on assay performance.
A one-step assay format achieves maximal sensitivity across a broad range of coating concentrations and at a lower concentration of conjugate than that in a two-step format.
The surfactants were tested at three concentrations: below, at, and above their Critical Micelle Concentrations (CMC) against 5 concentrations of E. coli BL21 in a three-step assay comprised of a 14 24 h turbidometric screen, a live-dead stain and viable colony counts.
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