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Match it up to the same position the last stencil was placed.
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The stencil is placed on the already inked plate and the colour is rolled onto the surface of the plate using a gelatin or soft rubber roller.
After detaching the PDMS substrate from the mold, the stencil is placed on commercial MEAs (MCS) and the stencil's pattern is aligned with the electrode locations.
A bovine serum albumin-coated (BSA-coated) stencil membrane was placed on a cell culture surface and C2C12 myoblasts were seeded on it.
Next, a clean glass slide was placed on the stencil and allowed to sit until the wax covered the entire interface between the stencil and the glass slide.
When the stencils had been placed into final position and weighted down with stones, Wu brought Cai an incense stick, which he held while she lighted it.
Instead of attaching the stencil to the wall, however, it will simply be placed over the surface of the item to be painted.
Unlike previous approaches, emphasis is placed on operating on a small stencil (at most four cells).
This stencil was preheated on a hot plate at 105 °C, and melted wax was spread over the stencil with a transfer pipet.
To pattern the wax on the slide, a stencil was made from silicone rubber (#31938707, MSC Industrial Supply Co).
If there is a breeze, or your stencil is very light, place a clear piece of glass on top of it to keep it from moving.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com