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The RNA extracted from a mixed tissue (leaf, root, stem bast and stem stick) and stem bast was used for RT-qPCR.
One paired-end (PE) cDNA library was generated from pooled equal quantities of total RNA from stem bast, stem stick, leaves, and roots.
To characterize the transcriptome of jute and obtain a number of genes associated with vegetative growth and development, total RNA was pooled from diverse tissues of leaves, roots, stem bast and stem stick at a vigorous vegetative growth stage.
Various tissues, including leaves, roots, stem bast, and stem stick, were collected from three plants on June 15th , 2013 which is a vigorous vegetative stage for fiber development in jute according to Xiong [ 1].
For one paired-end PE) cDNA library (left.fq and right.fq), which was generated from paired-end PEquantities of total RNA from stem bast, stem sticDNAlibrary and roots, the assembleft.fquence dand have been deposited at the NCBI Sequence rightArchive (SRA, http://www.fqbi.nlm.nih.gov/Traces/sra) under the accession number SRP060467 vide BioSamples SRS980707.
To identify the expression profile of the candidate genes associated with cellulose biosynthesis, the transcript abundance of all the candidate genes in the mixed tissue (leaf, root, stem bast and stem stick) and stem bast were investigated by using FPKM analysis (Fig. 8).
Similar(53)
After seeing the others chomp for a few minutes, Ibu had a rhubarb stem sticking out of his mouth.
One morning, we jumped up from our desks to run over to a milkweed stem stuck in a Mason jar on the plant table, where a monarch butterfly was emerging upside down from its oblong chrysalis, its wings still folded like a fan.
The sofa is $2,295 from Racing Furniture of High Point, N.C., which also makes a recliner called Daytona with a chrome-stemmed stick shift on the side for adjusting the seat.
I think the only reasonable way of resolving this contradiction is to accept that the tree is misrooted by extremely severe long-branch attraction, exactly as I explained before [ 1] (those long bare stems stick out like a sore thumb) and that the polarization by the insertion from eubacteria to neomura is correct; thus eubacteria are ancestral to neomura.
Let the stems stick out downwards to form one stem or holding point.
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