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Conclusions: We evaluated the utility of PDMS substrates for stem cell propagation and substrate mediated differentiation.
They provide a well-controlled environment for studying the process of stem cell propagation and differentiation.
However, stem cell propagation necessary to produce sufficient cell numbers currently utilizes fetal calf serum (FCS) as a growth supplement which may subsequently transmit animal pathogens.
Examples of the latter are presented, with special attention on the qualities of chitin that make it inherently suitable as scaffolds and matrices for tissue engineering, stem cell propagation and differentiation.
We reported that miR-34a negatively regulated cell proliferation, migration, and invasion and breast cancer stem cell propagation by downregulating Notch1.
Two strategies to interrogate the insulin growth factor 1 receptor (IGF-1R) pathway were investigated: vertical inhibition with dalotuzumab and MK-2206 oridaforolimusus to potentiate PI3K pathway targeting and horizontal cross-talk inhibition with dalotuzumab and MK-0752 to exert effects against cellular proliferation, angiogenesis, and stem cell propagation.
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From a different perspective, these data report on a safe approach to increase human neural stem cells propagation in culture, without altering their basic properties.
The metformin/AMPK action inhibits mTOR and cancer stem cell generation and propagation.
This review provides a perspective of both these cell types and discusses potential models of stem cell growth and propagation.
Bulge cells maintain their stem cell characteristics after propagation in vitro, thus ultimately they may be useful for tissue engineering applications.
Following in vivo knockdown experiments, this study concluded that HIFs were required for cancer stem cell survival and tumour propagation.
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