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Fatrai, S. et al. Mucin1 expression is enriched in the human stem cell fraction of cord blood and is upregulated in majority of the AML cases.
We also show that the forced expression of a CD22 RNA trans-splicing molecule (RTM) markedly reduces the capacity of the leukemic stem cell fraction of CD22ΔE12+ B-lineage ALL cells to engraft and cause overt leukemia in NOD/SCID mice.
In 2003 the first attempt was made to use a purified stem cell fraction [3].
Using this method each aspirated stem cell fraction developed into an embryoid body in these wells.
These findings suggest new approaches for directly targeting the stem cell fraction of human tumours.
Ectopic c-Myc expression in transformed human keratinocytes dramatically increases the cancer stem cell fraction and enhances tumorigenicity [12].
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It is clear that there are many gaps in our understanding about tubular, glomerular, and other speculated stem cell fractions in the kidney.
The purity of the basal stem cell populations remains a persistent problem due the difficulty of isolating pure (as opposed to enriched) stem cell fractions.
In vitro experiments showed non-correlation of stem cell fractions at confluence with initial proportions of stem cells, confirming the quorum sensing hypothesis.
However, one could argue that plerixafor mobilizes mostly the stem cell fractions that are responsible for short-term reconstitution of hematopoiesis, which could result in late graft failure.
For example, adult stem cells express similar cohorts of cell surface proteins, such as c-Kit, Sca-1 (stem cell antigen-1), CD34 and CD133; the presence of these antigens is used to isolate stem cell fractions with flow cytometry [ 52].
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