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One trigger for the study was the faking of stem cell data by South Korean researcher Woo Suk Hwang, which included cut-and-pasted cell images in a 2005 paper in Science.
At a press conference this morning, South Korean scientist Woo Suk Hwang apologized for publishing fabricated stem cell data in two Science papers in 2004 and 2005 but maintained that he was deceived by junior researchers and the victim of a conspiracy, according to Korean news reports.
Application to an existing neural stem cell data set demonstrates the method's applicability to highly complex systems and its ability to detect very low expression differences (<1.2-fold change), providing resolution which may be of significant interest in neural systems such as this.
To obtain diseased stem cell data, we identified a few studies with multiple datasets on myelodysplasia, acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) samples.
PAC managed the experimental stem cell data collection.
ROAST is the only existing software that could have been used for our mammary stem cell data example.
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Although Cdx4 possesses intrinsic ability to bind the CBSs, Cdx4 itself is not sufficient to initiate Hoxa9 expression in the embryonic stem cells (data not shown).
Furthermore, the removal of CD16 antibody by extensive washings of NK cells before their culture with monocytes and stem cells had similar results to those which were left unwashed and used in co-cultures with monocytes and stem cells (data not shown).
As expected, IL-2 treated NK cells lysed a much higher percentage of MSCs and DPSCs when compared to untreated NK cells, although a range of 20% to 70% cytotoxicity could be observed by untreated NK cells depending on the donor NK cells used to lyse stem cells (data not shown).
In particular, we also found that zinc stimulates osteoblastogenesis in human mesenchymal stem cells (data not shown).
CDC2 was negative in differentiated cells of neural stem cells, versus overexperssion in differentiated cells of brain tumor stem cells (data not show).
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