Your English writing platform
Discover LudwigSuggestions(5)
Exact(26)
A multipotent population of nontubular cells was isolated from the mouse kidney based on the expression of stem cell antigen-1 (Sca-1) and the absence of lineage (Lin) markers of bone-marrow-derived stem cells [112].
In contrast to stem cell antigen-1 Sca-1 -positive/CD90-negative Sca-1 -positive/CD90-negative Sca-1 -positive/CD90-negative Sca-1 -positive/CD90-negative
Stem cell antigen-1 (Sca-1) expression by the cells remained high after 7 days in culture (77±20% vs. 66±6% at day 0) while CD34 expression was reduced (19±12% vs. 61±10% at day 0) and myosin heavy chain expression was scarce (not quantified).
We found these GFP+ BMD cells to be positive for stem cell antigen-1 and c-kit, but negative for CD31 and CD34.
Viable transduced cells were further enriched for primitive hematopoietic cells by exclusion of cells expressing linage markers (Gr-1, B220, Ter-119, CD4, CD5 and CD8) and selection for cells expressing the stem cell antigen-1 (Sca-1).
MSC did not express CD11b, CD45, CD31, CD34, CD11c, Gr-1, c-kit, TER-119, B220, CD3, but express MSC-associated antigens such as Sca-1 (stem cell antigen-1) and CD90.2 (Figure 1A).
Similar(34)
Several laboratories have identified distinct populations of CPCs including side population (SP) cells [ 8], stem cell antigen-1-positive (Sca-1+) cells [ 9], c-kit-positive (c-kit+) cells [ 10], Islet-1-positive (Isl-1+) cells [ 11, 12], Wilms' tumor 1-positive (WT1+) epicardial progenitor cells [ 13], and cardiosphere-derived cells (CDCs) [ 14].
The following monoclonal antibodies directed against mouse surface markers anti-CD45 cytochrome C (CyC), anti-stem cell antigen-1 (Sca-1) phycoerythrin (PE), unconjugated anti-CD9, anti-CD3 CyC, anti-CD4 peridinin chlorophyll-alpha protein (PerCP), anti-CD73 PE were purchased from Pharmingen/Becton Dickinson.
Cell sheets composed of stem cell antigen-1 (sca-1) positive, or kit-positive cells may represent additional promising approaches.
Independent study by Burger et al. also identified a candidate population of PSCs in the proximal region of mouse prostatic ducts, using stem cell surface marker known as stem cell antigen 1 (Sca-1, also known as Ly6a) [ 48].
Stem cell antigen 1 (Sca-1), a member of the Ly6 family, is an 18 kD glycosyl phosphatidylinositol-anchored cell surface protein (GPI-AP) composed of a globular core, where the GPI anchor attaches, and three protruding finger loops (Gumley et al., 1995).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com