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The reddish flush on the stem apex is very odd (as is the entire fungus!).
Meanwhile the original tip zone continues to elongate, eventually surpassing the stem apex.
The zygote then divides, developing into an embryo, which in turn develops the first leaf, root, and stem apex.
Fern stems never become woody (composed of secondary tissue containing lignin), because all tissues of the plant body originate at the stem apex.
The leaf, rather than the stem apex, is the light-receiving organ in the photoperiodic reaction, although it is at the apex that subsequent developmental changes occur.
No cotyledon, stem apex, or root apex is organized in this early period; these organs do not appear until after germination has occurred.
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Total RNA was purified from stem apexes of three samples (Ari971, Ari1327 and Ari3697) using Trizol reagent (Invitrogen) according to the manufacturer's protocols.
The stem apexes of wild-type upland cotton (Ari971) and its dwarf mutant (Ari1327) and tall-culm mutant (Ari3697) at the fifth true leaf stage were extracted for RNA, respectively.
At the fifth true leaf stage, stem apexes of wild type and mutant seedlings were harvested and immediately frozen in liquid nitrogen and stored at −80 °C until further use.
In this study, we try to reveal the networks involved in regulation mechanism of plant height in Gossypium hirsutum by comparision analysis of transcriptome and small RNA sequencing of stem apexes at the fifth true leaf stage among the dwarf-mutant (A1), tall-mutant (A3) and their wild type (A9).
In particular, there is evidence for a predominance of radiolucent lines in the vicinity of the femoral-stem apex (zone 1 femoral) and the medial tibial plateau (zone 1 tibial).
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