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Paraffin embedded NPC sections were subjected to antigen retrieval by dewaxing for 15 min at 95°C in 0.01 M sodium citrate buffer in a steam cooker.
They were then submerged in citrate buffer (1.8mM Citric acid and 8.2mM Sodium citrate, pH = 10.6) and subjected to high pressure for 10min using a steam cooker.
Antigen retrieval was undertaken in a steam cooker for 40 min in Target retrieval solution (Dako, Glostrup, Denmark).
Endogenous peroxidase was blocked with 3% hydrogen peroxide; subsequently the sections were washed by distilled water and heated in a citrate buffer (pH 6.0) using a steam cooker at 95°C for 30 min.
Antigen retrieval was performed in a steam cooker (Biocarta Europe, Hamburg, Germany) for 5 min at 120°C using a target retrieval solution (pH 6) (TRS6, DakoCytomation) or 0.01 M Na-citrate buffer (pH 6).
The ensuing production process is automated and involves crushing the carcasses, followed by high temperature heating (steam cooker), and finally the separation of residue into sterile fat (tallow) and solids (meat and bone meal).
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This cooker unites the characteristics of reflector cookers, steam cookers, pressure cookers and heat accumulating solar cookers.
His dishes, likewise, are small, as are the kitchen (two hot plates, two steam cookers) and the restaurant itself, which suggests the experience of dining in a well-appointed subway car.
Today, far fewer Americans have experience making foods from scratch, and fewer still understand food preservation, which requires both skill and an investment in steam cookers, jars and lids, said Janet Poppendieck, a Hunter College sociologist who has studied emergency food needs.
The scent is heady and I am transported back to my childhood, watching my Amma shape a dough of rice into cups, filling them with the balls of coconut and jaggery and steaming the dumplings in steam cookers on the gas stove.
That was never likely to happen for Lingard on an afternoon where the air inside the stadium sat like a steam-cooker, the kind of drowsy, heavy heat that hits you in the chest and sinks down into the lungs.
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