We also point out that the steady state translation rate and protein concentration can be solved from the first method by simulating the translation process for a given initiation rate, determining the translation rate, then determining the protein concentration and iterating.
The consequence of these multiple expression states is that the steady state translation of transcripts from any low expression gene contributes fewer proteins than a high expression gene to the total cellular protein.
(2) k = 1 x d x d t = 1 P d P d t In the steady state translation output of a genome represented by Eq. (2), the protein output per unit total RNA is (3) T = k P R, where T = the protein translation output of a genome, measured in units of total protein, P, per unit total RNA, R, in a bacterium in steady state growth with instantaneous growth rate, k [ 12, 20].
In analogy to the steady-state reaction of an enzyme complex, we propose a steady-state translation complex for mRNA from any gene, and derive a maximum specific translation activity, T a (max), as a property of the ribosomal reaction complex.
The main effect of the Nod/Uv lesions on the interaural tVOR was to reduce sustained eye velocity during steady-state translation.
Thus, the insertions do not suppress NMD indirectly, by inhibiting steady-state translation for example.
Thus, NMD reduction is probably not due to less efficient steady-state translation of the mRNAs with the insertions.
Whether PYM remains bound to ORF57 during steady-state translation, rather than being recycled with EJC components following the pioneer round of translation will be of interest to determine.
Another possibility is that transcripts harboring early PTCs in the 5' region of the mRNA escape NMD detection and enter the steady-state translation pool; this occurs in mammalian cells [ 16].
In agreement with the mRNA quantifications, the reporter with the spacer yields more GFP, suggesting that lengthening the distance between stop codon and intron not only suppresses NMD, but it also does so without impairing steady-state translation efficiency.
