We confirm our analytical result that the method ensures that the damage-modified steady states remain steady states with 100% frequency.
Upon stimulation of both cell lines with GH, quantification of mature GHR levels showed that after 90 min about 50% of the GHRs were degraded in γ2A_GHR_Jak2 cells, while in γ2A_GHR cells GHR steady state levels remained unchanged (Fig. 6A).
At room temperature, 100 n m GABA caused a significant increase in RMS noise that quickly attained a steady state and remained stable for 20 30 min (Fig. 7A: normalized response, 233.9 ± 48.5% at 5 min after GABA application, 232.4 ± 98.4% at 25 min after GABA application, n = 6).
During the steady state period, the remaining working nodes perform the regular monitoring and communication.
In similarity with the methane conversion profiles, H2 formation rates exhibited quick steady state and thereafter remained invariant with time.
Steady state CO2 production remained constant when the trawling-induced physical mixing multiplier (PMM) varied from 0 to 10 (Fig. 5).
Importantly, even in the most unrealistic case of complete knockout without compensation and ignoring feedback inhibition, absolute steady state superoxide levels remained low, never rising above 0.5 nM.
In addition, we introduce a third time scale for the modelling output, the initiation of apoptosis and necrosis as a function of the steady state of the remaining signalling variables.
As the action potentials, Ca2+ spikes and muscle twitching ceased, a steady state tonic contraction remained, associated with a maintained, significant rise of baseline Ca2+ (68.3 ± 14.2%) of spontaneous Ca2+ response and membrane depolarisation which reached maximal level at this time.
The steady-state profiles remain to be collected at this stage of the research.
