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The assay is based on time-resolved measurements of the luminescence kinetics and implements multiple enzymes to convert PPi to ATP that is, in turn, utilized to produce light and to hydrolyze PPi for measurement of the steady state background luminescence.
In contrast, a steady state background of endogenous DNA lesions is present, which contribute to nonlinear dose-responses for mutations.
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One explanation for the lack of significant changes in VH level in this case was probably that the renal injury was not severe enough to cause sufficient changes compared to the background level of VH; the steady-state background level would correspond to 20 days accumulation of carbamoylated Hb in mouse; cf. [44].
So if there's "concern," it appears to be a steady-state background hum.
The consequence is a high steady-state background level of DNA damage, making collisions between the replication machinery and lesions in the template inevitable.
For a given flow rate and electrode depth, a steady-state background measurement was first obtained in oxygen-free ACSF (bubbled with 95%N2/5%% CO2); oxygen-saturated buffer (bubbled with 95%O2/5%% CO2) was then applied, and the increase in detected oxygen was recorded.
The analysis focuses on the propagation of disturbance waves on nonuniform steady-state backgrounds and takes into account the critical column-end effect observable only under nonequilibrium conditions.
0. The result is equivalent to the solution for the steady state coherent background.
Distributions of ORP data at steady state and background conditions were significantly different, but the DO distribution with an average of 5.93 mg/L and standard deviation of 0.09 and the range of 5.55 6.30 mg/L, was not statistically different than background (average 6.05 mg/L, standard deviation of 0.03, range of 5.93 6.17 mg/L).
Distributions of ORP data at steady state and in background conditions were separate and significantly different (p < 0.0001).
Distributions of both ORP and DO at a steady state and in background conditions do not overlap and are statistically different (p < 0.0001), but DO data distributions of the two groups, background conditions and steady state, are closer than previous experiments.
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