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This would generate a steady sampling of conformations at defined rates at equilibrium (Scheme 1).
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To ensure the steady state, sampling took place at least 24 h later (usually 1 to 2 days later, respective 4 to 5 days (t = 96 120 h) after starting continuous growth).
Both modeling approaches led to reliable prediction of transient and steady state sample temperature.
Steady state samples were collected after four complete volume changes at an OD600 of 34.
For the steady state samples, synchronized L1 stage animals were placed onto NGM plates containing 200 mM NaCl and allowed to grow to the young adult stage.
Steady state samples for extracellular metabolite analysis were collected after 3 volume changes.
From cultures which received 0.5% and 2.8% O2, two separate steady state samples were also analysed.
The average times for bilirubin baseline and steady-state sampling were 10 13 am (±1 38) and 09 44 (±1 22) am, respectively.
It takes some time until the concentration gradient in the surrounding tissue of the catheter has developed and a steady-state sampling takes place (Beneviste et al. 1989).
In addition, from one of the cultivations with 1.0% O2 in the feed gas, four separate steady state samples were analysed.
At the time of initial PK steady-state sampling, both patients were receiving liposomal amphotericin B (L-AmB) with one also receiving voriconazole at recommended maintenance doses (5 mg/kg and 4 mg/kg, respectively).
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