Sentence examples for steady binding from inspiring English sources

The resulting avidity effect, i.e., the combined synergistic binding of two Strep-tag®II moieties to tetrameric Strep-Tactin®, reduces the off-rate for more steady binding under non-competitive conditions.

The function of the steady binding partners was less obvious, but their stimulation-independent and non-dynamic association to SLP65 was consistent with the functional properties of a preformed BCR transducer complex that was predicted based on genetic evidence and explained the efficient coupling of BCR-proximal effector molecules (Wienands et al, 1996).

Steady state binding affinities were measured on a Biacore T100 instrument.

The steady-state and pre-steady-state binding events associated with biomolecular assemblies can be quantified with this technique, so long as the proper assays and binding models are developed.

Steady-state binding equilibrium analysis with conformation-dependent human H5N1 neutralizing MAb demonstrated that 293 Flp-In secreted H5N1 Handand HA0 proteins were properly folded (Fig. 2A).

For non-myristoylated ArfA, steady-state binding of GTPγS reached a stoichiometry of ∼1.6% of protein in the presence of LUVs (Fig. 1B triangles).

General fitting and steady-state binding affinity calculated with Biacore evaluation Version 3.0 software helped release a relative high binding affinity for tubulin with the dissociation constant (Kd) value of 11.1 µM, which was two-fold lower than that of colchicine (24.6 µM).

Assuming 10,000 receptors/cell [31] and that the receptors have achieved steady-state binding with a fixed Kd of 5 nM, then in the middle of the 0 1000 nM gradient the average concentration was 500 nM and the absolute slope was 2.5 nM µm−1.

Pre-steady-state binding experiments were performed using an Applied Photophysics SX20 stopped-flow spectrofluorimeter.

(E ) Quantification of steady-state binding of the indicated p62 variants to the indicated ubiquitin chains cross-linked to 2 µm latex beads.

(G ) Quantification of steady-state binding of the indicated p62 variants to beads coated with GST-mono-, di- or –tetra-ubiquitin.