Value is generated by steady, reliable associations with others.
Moreover, because CFP and YFP fusions generally retain activity and maintain their normal subcellular localizations, the detection of CFP YFP FRET in live cells typically reflects the steady-state association of functional proteins in their native states.
For each elongation, it was necessary to employ different concentrations of complementary strands to achieve steady-state associations, 100 nM (n), 150 nM (n + 1), 200 nM (n + 2), and 250 nM (n + 3 and n + 5).
When adding either the radioligand or the competitor, a steady insight into association, retention and dissociation of the radiolabelled ligand is possible during the experiment with high spatial and temporal resolution.
Thus GSK3 inhibition stabilizes steady state Axin APC2∆SAMPs association, as did deleting R2 or B, consistent with our model that phosphorylating these motifs normally antagonizes Axin Arm rpts association.
Finally, we detected steady ligands whose association to SLP65 was almost unaffected by BCR engagement in that it was already 'preformed' before BCR activation and remained unchanged thereafter.
Elizabeth Austerberry, chief executive of Moat, which also houses tenants across the southeast of England, said that rent was the biggest source of steady income for associations.
Landforms exposed for millions of years to a constant environment may display a climax (steady-state) landform association that is essentially timeless and in which landform evolution through denudation is reduced to mere negative allotropic growth.
"Our surveys tell us fund-raising has been holding steady," said Paulette V. Maehara, the association's president and chief executive, who emphasized that the findings for 2007 were preliminary and that in any case the environment could change over the course of 2008.
Our finding of a positive association between steady state FFA and plasma α HB concentrations in the whole cohort supports the possibility that an increased NADH/NAD+ ratio favors reduction of α KB to α HB (Figure 7).
