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The methylation status of sequences where CpG sites are spaced (e.g., only one may occur in any given fragment) may appear unmethylated even if the few CpG sites present are actually methylated.
A direct comparison of the inactivation status of sequences integrated into the same genomic location would permit a direct analysis of the impact of cis-acting sequences on XCI and potentially identify sequences containing escape elements, waystations, and/or boundary elements.
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The strategy and the status of sequencing and assembly are summarized in Fig. 1.
The subtelomeric region size indicated in the table corresponds to the whole unassigned contig sequence Figure 1 and Additional file 2 summarize the present status of sequence completion for each chromosome end.
A recent study by K ong et al. (2009), for example, illustrates that knowledge of the epigenetic status of sequence alleles (in this case, the parent-of-origin of the alleles) is often necessary to establish significant associations with phenotypes.
Methylation status analysis of sequences at the H19/IGF2 imprinted locus indicated that distinct epigenetic features characterize hMSC populations and condition the transcriptional effects of SYT-SSX expression.
Bisulphate sequencing and restriction digestion have also been used to analyse the methylation status of repeat sequences (such as L1 and IAP repeats) and some single-copy sequences (such as actin) (Howlett and Reik, 1991; Oswald et al., 2000).
However, under the current status of the sequencing technology, GS20 sequencing reads are limited to approximately 120 bases, thus in this experiment the full sequence (133 141 bp including primer, species dependent) was not returned and our analyses were limited to simply discriminating using the primer at the sequence end of the product.
Finally, the GenBank database was searched for nucleotide sequences with the names of the tentative potyvirus species listed by the ICTV [3] and the likely taxonomic status of those sequences was tested again following the procedure used for the unrecognised sequences.
Epigenetic regulation can be seen as a physical process preceded by the stochastic transcription of the appropriate coding sequences, dependent on the spatial ordering of the chromatin and the "status" of those sequences and contingent on the availability of the gene product precursors contained in multi-compartment post-translational steady states.
With the aims described, in the context of the current status of genome sequencing efforts, we sequenced the genome of the pearl oyster P. fucata.
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