Genes that fulfilled the criteria of technical, statistical and pathway analyses were validated by the quantitative reverse transcription PCR-based TaqMan Low Density Array (Applied Biosystems) system, according to the manufacturer's protocol.
DCE analyses were validated using Stata 14.1.
All statistical analyses were performed using a validated statistical software, Stata Version 10.0.
Differentially expressed genes were identified using statistical analyses, and were validated with external databases and with immunohistochemistry.
Statistical analyses were conducted to further evaluate the model performance and validate the reliability of the proposed method.
Statistical analyses were also performed, as previousely described by [ 42] and [ 43] to validate the expression profile obtained.
Statistical analyses were performed using GraphPad Prism.
Statistical analyses were performed using STAMP26.
All statistical analyses were performed in R50.
Statistical analyses were performed initially by ANOVA.
