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Cell growth increased slightly during the stationery phase between 20 and 30 days.
This species then undergoes mass transfer with the stationery phase and ideally leads to the separation of the components.
In spite of expression of SAM 2 gene up to 90 h, S-adenosylmethionine accumulation tended to plateau after 72 h, presumably because of the limited ATP available in the cells at stationery phase.
Cells were grown from frozen stock in media at running temperature to stationery phase.
Before loading onto the device, cells were grown from a frozen stock to stationery phase.
Cells were first cultured in YE media at 26°C overnight until late stationery phase, then transferred to EMM media containing a nitrogen source and incubated for 24 hr until mid-log phase (OD = 0.5 0.7).
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